DEPARTMENT OF BIOCHEMISTRY AND BIOTECHNOLOGY
Annamalai University
Annamalainagar-608 002.
Tamilnadu, India
NATIONAL WORKSHOP ON
“ANIMAL CELL CULTURE TECHNIQUES”
(ACCT-2011)
March 18-20, 2011
Host Department
The Department of Biochemistry was started in 1994. In a short span of time the Department has made rapid strides in teaching and research activities. The Department offers 2 year M.Sc. Biochemistry, M.Sc. Biotechnology programs and a 5 year integrated M.Sc. Biotechnology program. In addition, M.Phil and Ph.D. programs are offered. The major areas of research in the Department include free radicals and antioxidant defence systems, analysis of medicinal utilities of natural products for the treatment of diabetes, heart disease, hepatotoxicity, cancer and for radioprotection. The Department has collaborative programs with a number of national and international universities and institutes. The Department has organized number of workshops and conferences where participants have come from different institutes and universities. DST has recognized the department for funding under DST-FIST program and UGC has recognized the department for Phase II level SAP-DRS program.
Workshop Background
Cell culture has become one of the major tools used in life sciences today. Cell cultures provide a good model system for studying 1) basic cell biology and biochemistry, 2) the interactions between disease-causing agents and cells, 3) the effects of drugs on cells and 4) the process and trigger for aging. Liver and kidney-derived cell lines are widely used alone or in
conjunction with animal tests to study the effects of new drugs, cosmetics and chemicals on survival and growth in a wide variety of cell types. Since both normal cells and cancer cells can be grown in culture, the basic differences between them can also be closely studied. In addition, it is possible, by the use of chemicals, viruses and radiation, to convert normal cultured cells to transformed cells. Cultured cancer cells also serve as a test system to determine suitable drugs and methods for cancer treatment. One of the earliest and major uses of cell culture is the replication of viruses in cell cultures (in place of animals) for use in vaccine production. Cell cultures are also widely used in the clinical detection and isolation of viruses, as well as basic research into how they grow and infect organisms. Cell-based assays have become increasingly important for the pharmaceutical industry, not only just for cytotoxicity testing but also for high throughput screening of compounds that may have potential use as drugs. In this background the present workshop is designed to provide a hands-on training in basic animal cell culture techniques and to emphasize application of animal cell culture techniques in anticancer drug screening.
Program Schedule
Morning session: 9.00 am to 1.00 pm (with a 15 min tea break)
Lunch: 1.00 pm to 2.00 pm
Afternoon session 2.00 pm to 6 pm (with a 15 min tea break)
Workshop Program
Besides hands-on training, participants will be encouraged to carry out the techniques themselves. The workshop will be conducted for 15 participants. This cell culture workshop will
cover lectures and hands-on experience on
Basics in animal cell cultivation
- — Media preparation
- — Sub-culturing
- — Lymphocyte culture set-up
- — Assessing viability of cultured cells
- — Suspension and adherent culture
- — Cryopreservation and thawing
- — Dealing with HeLa cell cross contamination
Testing cytotoxicity of anticancer agents
- — MTT assay
- — Clonogenic cell survival assay
Evaluation of genotoxicity of anticancer agents
- — Alkaline single cell gel electrophoresis & Analyzing comet attributes by CASP software
- — Cytokinesis blocked micronuclei assay (CBMN cytome)
Analysis of apoptotic morphological changes by
- — Ethidium bromide/acridine orange staining
- — DAPI staining
Measurement of intracellular ROS (DCFH-DA staining)
Assessment of mitochondrial membrane potential
(Rhodamine 123 staining)
Venue
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar - 608 002, Tamilnadu.
Registration
Participants are requested to apply for registration in the prescribed format along with a demand draft for Rs. 3,000/- drawn in favour of “The Registrar, Annamalai University, payable at Chidambaram, Tamilnadu. Registration fee includes accomodation, food, workshop manual and kit.
TA/DA and Accommodation
TA/DA will be given to 10 participants on first come first served basis. Accommodation for the participants will be provided in University Guest House/Hostels.
Time Schedule
Last date to receive application: 1st March, 2011. Upon receipt of the completed application form, the confirmation of registration will be intimated to the participants by email.
The filled in application along with DD should be sent to
Dr. N. Rajendra Prasad
Organizing Secretary
National Workshop on “Animal Cell Culture Techniques”
Department of Biochemistry & Biotechnology
Annamalai University
Annamalainagar 608 002.
Tamilnadu.
Phone : 04144-239141
(0)9842305384
E.mail : drprasadnr@gmail.com
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